Two approaches that come to mind:
>>> df
A B C D
0 0.424634 1.716633 0.282734 2.086944
1 -1.325816 2.056277 2.583704 -0.776403
2 1.457809 -0.407279 -1.560583 -1.316246
3 -0.757134 -1.321025 1.325853 -2.513373
4 1.366180 -1.265185 -2.184617 0.881514
>>> df.iloc[:, 2]
0 0.282734
1 2.583704
2 -1.560583
3 1.325853
4 -2.184617
Name: C
>>> df[df.columns[2]]
0 0.282734
1 2.583704
2 -1.560583
3 1.325853
4 -2.184617
Name: C
Edit: The original answer suggested the use of df.ix[:,2]
but this function is now deprecated. Users should switch to df.iloc[:,2]
.